Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: SOX2OT is increased while miR-194-5p is decreased in GC tissues and cell lines. Relative expression of SOX2OT in (A) GC tissues compared with healthy tissues and (B) GC cell lines compared with normal gastric cell line was detected by RT-qPCR. Relative expression of miR-194-5p in (C) GC tissues compared with healthy tissues and (D) GC cell lines compared with was detected by RT-qPCR. All data are presented as the mean ± standard deviation from three independent experiments. **P<0.01, ***P<0.001 compared with healthy tissues or GES-1 cells. SOX2OT, SOX2 overlapping transcript; miR, microRNA; GC, gastric cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: Knockdown of SOX2OT suppresses cell proliferation of GC cells. MKN-74 and MGC-803 cells were transfected with sh-SOX2OT or sh-NC respectively. (A) Relative expression of SOX2OT was detected through RT-qPCR and (B) cell proliferation was detected through CCK-8 assay in MKN-74 cells. (C) Relative expression of SOX2OT was detected through RT-qPCR and (D) cell proliferation was detected through CCK-8 assay in MGC-803 cells. All data are presented as the mean ± standard deviation from three independent experiments. *P<0.05, **P<0.01 compared with sh-NC group. SOX2OT, SOX2 overlapping transcript; GC, gastric cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CCK-8, Cell Counting Kit-8.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: Knockdown, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: Knockdown of SOX2OT suppresses cell mobility of GC cells. MKN-74 and MGC-803 cells were transfected with sh-SOX2OT or sh-NC respectively. (A) Cell invasion and (B) cell migration abilities were detected using a Transwell model assay in MKN-74 cells. (C) Cell invasion and (D) cell migration abilities were detected using a Transwell model assay in MGC-803 cells. All data are presented as the mean ± standard deviation from three independent experiments. **P<0.01 compared with sh-NC group. Scale bar, 100 µm. SOX2OT, SOX2 overlapping transcript; GC, gastric cancer.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: Knockdown, Transfection, Migration, Standard Deviation

Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: Knockdown of SOX2OT inhibits EMT in GC cells. MKN-74 and MGC-803 cells were transfected with sh-SOX2OT or sh-NC respectively. (A) Representative western blot and (B) quantification of E-cadherin, N-cadherin and Vimentin expression in MKN-74 cells. (C) Representative western blot and (D) quantification of E-cadherin, N-cadherin and Vimentin expression in MGC-803 cells. All data are presented as the mean ± standard deviation from three independent experiments. **P<0.01, ***P<0.001 compared with sh-NC group. SOX2OT, SOX2 overlapping transcript; EMT, epithelial-mesenchymal transition; GC, gastric cancer.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: Knockdown, Transfection, Western Blot, Expressing, Standard Deviation

Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: miR-194-5p is a target of SOX2OT and miR-194-5p inhibitor counteracts the effects of SOX2OT shRNA on GC progression. MKN-74 cells were transfected with sh-SOX2OT, sh-SOX2OT + inhibitor NC, sh-SOX2OT + miR-194-5p inhibitor respectively. (A) Target sequences of miR-194-5p in SOX2OT mRNA were analyzed through bioinformatics. (B) Relative expression of miR-194-5p was detected through RT-qPCR (**P<0.01 compared with mimic NC or inhibitor NC group). (C) The interaction between SOX2OT and miR-194-5p was confirmed by luciferase reporter assay (**P<0.01 compared with SOX2OT WT group). (D) Relative expression of miR-194-5p was detected through RT-qPCR. (E) Cell proliferation was detected through CCK-8 assay. (F) Cell invasion and (G) migration abilities were detected using Transwell model assay. (H) Representative western blot and (I) quantification of E-cadherin, N-cadherin and Vimentin. All data are presented as the mean ± standard deviation from three independent experiments. **P<0.01 compared with control group, #P<0.05, ##P<0.01, ###P<0.001 compared with shRNA + inhibitor NC group. SOX2OT, SOX2 overlapping transcript; miR, microRNA; GC, gastric cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CCK-8, Cell Counting Kit-8.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: shRNA, Transfection, Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay, CCK-8 Assay, Migration, Western Blot, Standard Deviation, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Oncology Letters

Article Title: The SOX2OT/miR-194-5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial-mesenchymal transition

doi: 10.3892/ol.2018.9433

Figure Lengend Snippet: SOX2OT/miR-194-5p axis suppresses GC tumor growth and metastasis through inhibition of EMT in vivo. sh-NC or sh-SOX2OT transfected MKN-74 cells were injected into BALB/c nude mice, respectively. (A) Tumor volumes were measured every 5 days. (B) Relative expression of miR-194-5p was detected through RT-qPCR. (C) Representative western blot and (D) quantification of MMP-2 and MMP-9. (E) Representative western blot and (F) quantification of E-cadherin and Vimentin. All data are presented as the mean ± standard deviation from three independent experiments.*P<0.05, **P<0.01 compared with sh-NC group. SOX2OT, SOX2 overlapping transcript; miR, microRNA; GC, gastric cancer; EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MMP, matrix metalloproteinase.

Article Snippet: Cell transfection Short hairpin RNA (shRNA) against SOX2OT and their negative control (sh-NC), miR-194-5p mimic or inhibitor and their negative controls (mimic NC, inhibitor NC) were synthesized (GenePharma, Shanghai, China).

Techniques: Inhibition, In Vivo, Transfection, Injection, Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction